OUR SERVICES
AT THE HEART OF A CORETECH
Network of 18 platforms for researchers, clinicians and pharmaceutical companies
List of services offered by the platform:
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Experimental protocol design and realization*
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Training in organoid cultures: create, expand, analyze and cryopreserve organoids*
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Culture of organoids (L2) from normal, tumoral, mouse or human tissues in two different techniques (in a Matrigel drop, or on top of a collagen-coated filter) depending on the user goal*.
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Culture in special chamber if the user wants to analyze on his own by whole mount immunostaining*
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Expansion of organoid culture after enzymatic dissociation for amplification*
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Kinetic imaging of culture in brightfield or GFP/RFP filters*
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Fixation and inclusion for future histological analysis by the user*
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Freezing of organoids for future culture experiment by the user*
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Manipulation of organoids include: lentiviral infection, electroporation*
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Drug testing and analysis of the sensitivity of the drug using viability test with CellTiter Glo Assay or RealTime-Glo MT Assay or cytotoxicity test using CytoTOX-Glo assays*
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Quantification and analysis of organoids using the Celleste software**
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Transparization for whole mount and analysis by the user with light sheet microscopy*
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Culture of normal and tumoral organoids with long-term conservation of immune cells**
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Constitution of clinically annotated biobanks from patient-derived organoids from rectum, colon and livers cancers**
Quotations and prices are available on request from the platform.
* list of available services
** in development, will be in service later