Pubmed

• Epithelial-derived basement membranes help orchestrate crypt-villus patterning in vitro 18 novembre 2025
  Intestinal organoid culture has widely depended on exogenous laminin-rich matrices to provide a supportive environment for organoid development. In this issue of Developmental Cell, Chrisnandy and Lütolf now report that human and mouse intestinal epithelial cells can secrete their own basement membrane, enabling organoid formation even without exogenous laminin.
  Janny Pineiro-Llanes
• Mechanical strain exacerbates Pseudomonas infection in an organoid-based pneumonia-on-a-chip model 18 novembre 2025
  No abstract
  Karen Hoffmann
• Injury and inflammation promote cancer progression at the anorectal junction 14 novembre 2025
  In anorectal cancer, epithelial tumors frequently develop in transition zones (TZs) between the anal and the rectal epithelia, a region subjected to inflammation and wounds. However, whether inflammation and wounds contribute to tumor development in the anorectal region remain totally unknown. Using mice with KRAS^(G12D) mutation selectively at the TZ cells, we found that recurrent […]
  Chloé Petitpas
• Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer 14 novembre 2025
  While dual KRAS and epidermal growth factor receptor (EGFR) inhibition shows promise in treating KRAS-mutant colorectal cancer (CRC), resistance remains a major challenge. Using genetically engineered mouse models, patient-derived organoids and xenografts, as well as clinical specimens, we discover that colorectal tumors surviving combined KRAS and EGFR inhibition acquire a Paneth-like cell state-a secretory lineage […]
  Yuetong Zhang
• Regulation of corneal stromal cell behavior by modulating curvature using a hydraulically-controlled organ chip array 12 novembre 2025
  Corneal curvature abnormalities drive ectatic diseases, yet their mechanobiological effects on stromal cells remain poorly understood. We developed a hydraulically controlled curvature array chip recapitulating disease-relevant geometries (33-56D) to investigate how keratocytes, fibroblasts, and myofibroblasts respond to geometric stress. Curvature-induced mechanical stress triggered dramatic cellular remodeling keratocytes exhibited significant proliferative enhancement and phenotypic...
  Minju Kim
• iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion 12 novembre 2025
  The number of spatial omics technologies being developed is increasing¹. However, a missing tool is one that can locate proteins in tissues in an untargeted manner at high spatial resolution and coverage. Here we present in situ imaging proteomics via expansion (iPEX), which integrates isotropic tissue magnification² with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. […]
  Fengxiang Wang
• Targeting polyamine metabolism and ferroptosis enhances the efficacy of KRAS-targeted therapy depending on KEAP1 status 11 novembre 2025
  The resistance to KRAS-targeted therapies, particularly due to co-occurring gene mutations, remains a significant challenge. Through a metabolite library screening, we reveal that polyamines sensitize KRAS inhibitors only in KRAS^(MU)/KEAP1^(WT) cells but not in KRAS^(MU)/KEAP1^(MU) cells. Transcriptome sequencing and metabolome profiling pinpoint SAT1, the key enzyme in polyamine metabolism, as essential for this divergence. In […]
  Yunyi Bian
• Patient-derived organoids to study glycosylation dynamics during gastric disease 9 novembre 2025
  Aberrant cellular glycosylation is a key event that accompanies and actively sustains gastric neoplastic transformation. Patient-derived organoids (PDOs) have recently emerged as promising ex vivo models to study human gastric diseases; however, their glycosylation landscape remains unknown. To evaluate gastric PDOs as avatars of in vivo tissue glycosylation, a biobank of gastric PDOs (n = […]
  Liliana Santos-Ferreira
• Injury-induced Clusterin⁺ cardiomyocytes suppress inflammation and promote regeneration in neonatal and adult hearts by reprogramming macrophages 8 novembre 2025
  Adult hearts scar after injury, while neonatal hearts regenerate. The mechanisms underlying this dichotomy remain unclear. Through comparative spatiotemporal single-cell analyses and dual recombinase-mediated lineage tracing, we uncovered an injury-induced Clusterin^(+) cardiomyocyte (Clu^(+) CM) population that coordinates reparative, anti-inflammatory macrophage activity. Following injury, Clu^(+) CMs emerge in the border zone of regenerative hearts but are […]
  Lei Fan
• The need for a global effort to attend to human neural organoid and assembloid research 6 novembre 2025
  A continuing international process is needed to monitor and advise this rapidly progressing field.
  Sergiu P Pașca